THE INVESTIGATION ON THE EFFECT OF STOMACH SECRETION

During the tests on white rats, which weight 170 – 200 g, the diluted extract was used (1:100). After giving this diluted extract (20 – 60 mcl / for the weight of 100g) to the animals, it was established that the secretion of gastric juice increased gradually to 10 ml. The secretion does not increase when giving bigger dosage of preparation (see 1 picture). The observation of the salt acid secretion showed that the largest amount of it was obtained when the diluted extract draught was 40 mcl / for the weight of 100 g (see 2 picture). The secretion of pepsin is largest when 60 mcl / 100 g (see 3 picture) draught is used. Observing an activity of lipazion (see 4 picture) and proteazion (see 5 picture), it was estabished that the largest activity of those ferments is when 60 mcl / 100 g draught is used. In this case the activity suddenly rises until after 6 hours it reaches the maximum point; later on it gradually decreases until it reaches the previous point

DETERMINATION OF LD₅₀ AND HYPERSENSIVITY (ALLERGY) EFFECT

18 –22 g of white mouse was investigated. Some extract was injected into the cavity of stomach and some was poured through probe into the stomach of the mouse. The facts were compared with the influence of 40 % etanol used in this case as ekstrahen. The experiment shown that LD₅₀ of the extract is 1.103 ml (55.15 ml/ for kg of weight), that is, 0.034 ml (1.7 ml/ for kg of weight) bigger then LD₅₀ of observed 40 % etanol, which is 1.069 ml (53.45 ml/ for kg of weight). The conclusion was drawn that the herbs of the extract do not increase the toxicity in comparison with the 40 % etanol.

As to hypersensivity (allergy) caused by the extract, the investigation on an active and passive anaphilacsation and the local hypersensivity were carried out. 250 – 300 mumps and 2500 – 3000 rabbits took part in the investigation. In order to have a larger number of allergens a half of LD_50, that is, 0.22 ml of the extract was used, which was diluted with physiological solution (1 : 4), in order not to have side effect caused by etanol. During the investigation nor active or passive anaphilacsation neither anaphilacsation shock was observed. The conclusion was made that in the place where the preparation was injured an allergic inflammation was not found.

DETERMINATION OF LOCAL EFFECT FOR SKIN AND MUCOUS MEMBRANE

There were investigated 2500 – 3000 rabbits and 300 – mumps. Not diluted extract, diluted extract (1: 1) and for control 40 % etanol were used for samples. Observing the effect of samples for skin and mucous membrane, histological changes were certified depending on the time of their appearance and heaviness of injury. The changes of epiderm, derm, hypoderm and epitel of mucous membrane, mucous membrane and under mucous membrane lamella were evaluated to a certain degree. The data of experiment shown, that the local effect for the skin and mucous membrane depends on the effect of local etanol. The least effect was using diluted extract ratio (1: 1). Using extract some histological changes, in comparison with the effect of 40 % etanol, appear later, that’s why they decrease the effect of the active substance of etanol extracted from the herbs for the skin and mucous membrane

DETERMINATION OF EFFECT FOR ALIGMENTARY CANAL (TRACT)

The patients of 41 – 82 age were investigated. The endoscopic investigation of gullet, stomach and duodenum were done for 83.3 % of them, the radiologistic investigation of stomach for 5.5 % of them. During the investigation it was diagnosed atrophy gastrit for 55.5 % of patients and superficial gastrit for 33.3 % of patient. The complains of patients were following: the feeling of fullness in the area of stomach (83.3 %), a stomachache (72.2 %), bad appetite (72.2 %), sicken (44.4 %), a whiff of stomach (27.7 %), constipation (27.7 %), vomiting (16.6 %), heartburn gnaw (11.1 %), throw up (5.5 %), diarrhoea (5.5 %). All patients had taken 30 drops of (1.5 ml) of the extract tree times a day 15 – 20 min before the meal. Two weeks later all indisposition of 66.6 % of patients disappeared and 33.4 % of patients decreased. After the investigation the rest of complains were a stomachache (11.1 %), sicken (11.1 %), a whiff of stomach (5.5 %), a constipation (5.5 %). During the treatment other different kind of effects were observed as well: mood improvement for 33.3 % of patients, sleepiness appearance for 16 % of patients, calming effect for 11.1 % of patients, improvement of sleep and disappearance of head swimming for 5.5 % of patients.

Making generalizations about investigation it was concluded that herbs used in making the extract (that is, the same preparation) improve digestion.